Fig. 3.

aRF1·aEF1α·GTP complex model and interactions between aRF1 and aEF1α. (A) A docking model of the aRF1·aEF1α·GTP complex. aEF1α is colored red (domain 1), orange (domain 2), and yellow (domain 3); aRF1 is colored as in Fig. S3. The bound GTP is shown in a green-colored stick model. The Mg²⁺ is shown as a magenta sphere. The five interaction sites between aRF1 and aEF1α are indicated, and A–D for domain B of aRF1 (red), and site 1 for domain C of aRF1 (green) are boxed. Domain B (aRF1) interactions with aEF1α are observed at four sites: A, switch I region; B, switch II region; C, domain 2 cleft region; D, domain C linker helix region. (B) The interaction sites are shown with the relevant amino acid residues depicted by stick models, colored red for those of aEF1α, and black for aRF1. The GGQ motif is modeled according to human eRF1 structure. Figures were rendered using Pymol (32).