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. 2010 Oct 25;107(45):19320–19325. doi: 10.1073/pnas.1012044107

Fig. 2.

Fig. 2.

SERCA2b increases resistance of cells to ER stress. (A) qPCR analysis of the mRNA levels of SERCA2b in the Ad-LacZ and Ad-SERCA2b-infected cells. (B) SERCA2b immunoblotting in the whole lysates of MEFs infected with Ad-LacZ or Ad-SERCA2b. Tubulin was used as a control. (C) MEFs was infected with Ad-LacZ or Ad-SERCA2b for 12 h and treated with thapsigargin (0.5 nM) for 0, 1.5, and 3 h. Immunoblotting was performed for phospho-PERKThr980, SERCA2b, and tubulin levels. (D) Ad-LacZ or Ad-SERCA2b-infected MEFs were stimulated with tunicamycin (0.0625 μg/mL) for 0, 0.5, 1, 1.5, 2, 2.5, and 3 h, and total lysates were blotted against phospho-PERKThr980, SERCA2b, and tubulin.