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. 2010 Oct 25;107(45):19231–19236. doi: 10.1073/pnas.1013162107

Fig. 4.

Fig. 4.

miR-1 and miR-133a associate with the Hand2 3′UTR simultaneously in primary cardiomyocytes. (A) Schematic diagram of the sequential pulldown of biotinylated miR-1 and miR-133a with the Hand2 3′UTR. (B, Left) In the absence of biotinylated miR-1 (no miR-1-B), no microRNAs are purified in the presence of the wild-type Hand2 3′UTR (WT UTR). (Middle) In the presence of miR-1-B and WT UTR, miR-133a is enriched, but not miR-20a or miR-26a (two cardiac microRNAs not predicted to bind to the Hand2 3′UTR). (Right) Sequential pulldown using the miR-133a double MRE mutant did not copurify miR-133a. Results represent two independent experiments.