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LC profiles of PA-labeled sialyl glycopeptides enriched from mouse serum before (top) and after (bottom) the treatment with PNGase F. A, LC pattern monitored by fluorescence detector. B, LC pattern monitored by MS detector. C, MS/MS spectrum of PA-labeled sialyl glycopeptide from α1-acid glycoprotein 1 (NLINDTIELR) detected at 45.9 min. D, MS/MS spectrum of the corresponding de-N-glycosylated peptide identified as NLIDDTIELR detected at 49.1 min.
