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. 2010 Nov;9(11):2517–2528. doi: 10.1074/mcp.M110.001719

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — Separation and identification of human epididymal proteins by 2D-PAGE and MALDI-MS Spectra. Reference maps of (A) the epididymal tissue pellet; (B) the epididymal fluid; example spectra of (C) HEL-S-162eP, (D) HEL-S-128m. The MS map (C, upper panel) and MS/MS map (C, lower panel) marked with b ions and y ions for glyceraldehyde-3-phosphate dehydrogenase identification. The sequence of precursor at m/z 1530.78 was analyzed by MS/MS to be VPTANVSVVDLTCR and the protein identified as glyceraldehyde-3-phosphate dehydrogenase. The MS map (D, upper panel) and MS/MS map (D, lower panel) marked with b ions and y ions for peroxiredoxin 6 identification. The sequence of precursor at m/z 1530.78 was analyzed by MS/MS to be LPFPIIDDR and identified as peroxiredoxin 6.