Fig. 10.

Potential disruption of proteins involved in nucleocytoplasmic trafficking in HRSV-infected cells. A, a diagrammatic representation of the nuclear pore complex showing examples of the nuclear pore proteins whose abundance was altered in the quantitative proteomic analysis of HRSV-infected versus mock-infected cells and also nucleocytoplasmic trafficking proteins. Many of the nups interact with one another and other nuclear envelope proteins; for example, nup53 interacts with nup98 and lamin B. B, indirect immunofluorescence confocal microscopy analysis of nuclear protein lamin B subcellular localization in mock- and HRSV-infected A549 cells 24 h postinfection. Lamin B proteins are stained red, HRSV proteins are shown in green, and the nuclei are stained blue with DAPI. A merge image is also presented. Scale bars, 20 μm. RSV, respiratory syncytial virus.