Fig. 12.

Indirect immunofluorescence confocal microscopy analysis of ND10s in HRSV-infected versus mock-infected cells at 24 h postinfection (A) and 36 h postinfection (B) is shown. PML proteins (the major constituent of ND10s) are stained red, the HRSV proteins are green, and the DNA in nuclei is stained blue with DAPI. A merge image is also presented. C, direct immunofluorescence confocal microscopy analysis of the subcellular localization of recombinant FLAG-tagged PML isoforms PML-I and PML-II in mock- and HRSV-infected A549 cells 24 h postinfection. PML-I and PML-II proteins are red, the HRSV proteins are green, and the nuclei are stained blue with DAPI. Scale bars, 20 μm. RSV, respiratory syncytial virus.