Table I. Parameters of magnetic bead handler protocol for biotin-PE immunoassay.

Step	Plate content	Volume	Mixing parameters
			Time	Temperature	Speed
		μl
1) Transfer	Magnetic bead array	50
2) Analyte capture	Sample	100	30 min	RT	750 rpm
3) Wash + transfer	Wash buffer	100	20 s	RT	Slow
4) Wash + transfer	Wash buffer	100	20 s	RT	Slow
5) Bead release to assay plate	Assay buffer	100	20 s	RT	Slow