Table III. Ambient analyte theory: repetitive measurement of biotin-PE from same sample.
Shown are the results for five sequentially performed analyses of biotin-PE from the same sample from the same well. The data from the first measurement were used for a five-parametric logistic fit. Using the parameters of this fit, the raw assay data were transformed to analyte concentrations. After five assays, an analyte decrease of 20% was observed in total, suggesting a captured amount of less than 5% per assay. AU, arbitrary units.
| Biotin-PE | First measurement |
Second measurement |
Third measurement |
Fourth measurement |
Fifth measurement |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | %CV | Mean | %CV | Mean | %CV | Mean | %CV | Mean | %CV | |
| pm | AU | AU | AU | AU | AU | |||||
| Blank | 8 | 8 | 8 | 16 | 8 | 8 | 8 | 13 | 8 | 13 |
| 1 | 82 | 2 | 82 | 3 | 80 | 1 | 72 | 3 | 70 | 2 |
| 5 | 315 | 2 | 309 | 1 | 293 | 1 | 271 | 1 | 253 | 3 |
| 20 | 1139 | 2 | 1158 | 3 | 1071 | 0 | 1015 | 1 | 981 | 3 |
| 80 | 3466 | 2 | 3580 | 1 | 3366 | 1 | 3211 | 2 | 3131 | 1 |
| 300 | 6181 | 1 | 6191 | 1 | 5996 | 0 | 5926 | 2 | 5895 | 1 |
| pm | %CV | pm | %CV | pm | %CV | pm | %CV | pm | %CV | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 2 | 1 | 3 | 1 | 1 | 1 | 3 | 1 | 2 |
| 5 | 5 | 2 | 5 | 1 | 5 | 1 | 4 | 1 | 4 | 3 |
| 20 | 19 | 2 | 20 | 3 | 18 | 0 | 17 | 1 | 17 | 3 |
| 80 | 78 | 2 | 83 | 1 | 75 | 1 | 69 | 2 | 67 | 1 |
| 300 | 312 | 1 | 314 | 1 | 276 | 0 | 265 | 2 | 260 | 1 |