Skip to main content
. 2010 Oct 29;132(46):16526–16532. doi: 10.1021/ja106552p

Figure 2.

Figure 2

Effect of GSH on the reductive nitrosylation of metMb (50 μM) by (A) 2 (100 μM), (B) 1 (100 μM), (C) DEA/NO (50 μM), or (D) Angeli’s salt (100 μM). The assay was performed in PBS (pH 7.4) containing 50 μM DTPA and 250 μM GSH at 37 °C under deaerated conditions. Scans are plotted at 0.5 and 60 min in panel A, 2, 4, 6, 9, 14, 23, and 56 min in panel B, and at 1-min intervals to 8 min and then at 11, 14, 18, 23, 28, and 40 min in panel C. Panel D includes spectra at 500 s (green trace), where the decomposition of Angeli’s salt was complete, and at 60 min (blue trace). The effect of GSH appears to be dependent on the batch of myoglobin, indicating the possible interaction with a contaminating protein such as Cu, Zn superoxide dismutase (data not shown; product formation is variable).