Figure 1.
ihog and boi mutants. (A, B). Illustration of the intron-exon structure of the ihog and boi loci, with coding sequences shaded black. (A) The sites of pBac insertions used to create ihogDC1 are indicated (triangles), as is the extent of the 3,479-bp ihogDC1 deletion (dashed line). (B) An EP insertion (triangle) was used in a screen to isolate the nonsense boiC1 mutation. The location and orientation of the zeste gene within an intron of the boi gene are indicated. (C) Schematic diagram showing the protein structure common to both Ihog and Boi. The boiC1 mutation is predicted to truncate a significant portion of Boi (grey), including the second FN3 domain, transmembrane domain and cytoplasmic tail. (D-F) Whole mount in situ hybridization to third instar (L3) eye imaginal discs (white bracket) and connected brain hemispheres (black bracket). (D,E) In wild type (WT), ihog and boi transcripts are enriched in eye discs, where they appear to have overlapping expression. (F) ihog expression is not detectable in ihogDC1 null mutants.