Skip to main content
. 2010 Oct 6;4:135. doi: 10.1186/1752-0509-4-135

Table 3.

Simulated metabolic fluxes corresponding to the best fit presented in Table 2.

flux Control Edelf: 0.5 μg/mL Edelf:1 μg/mL
hk***
0.0901118
1
0.12
1
0.135
1
pfk
0.0968532
1.07481
0.120985
1.00821
0.135572
1.004237
fbpase
0.00716729
0.07954
1.46E-3
0.01219
0.0009715
0.007197
pep- > g3p
0.00694225
0.07704
0.00749499
0.06246
0.0025560
0.018933
g3p- > pep
0.186074
2.06492
0.246177
2.05148
0.271442
2.010681
pk
0.181224
2.01110
0.253755
2.11463
0.28068
2.079111
TCA- > pyr
2.23E-3
0.02470
0.0152166
0.12681
0.0118396
0.087701
lac- >
0.12219
1.35598
0.153661
1.28051
0.128901
0.954822
pdh***
0.057984
0.64347
0.0815881
0.67990
0.140089
1.037696
pc
1.05E-3
0.01165
0.0185053
0.15421
0.0116895
0.086589
CitSyn**
0.0351159
0.38969
0.0547404
0.45617
0.120038
0.889170
cit- > mal**
0.0366362
0.40656
0.0549581
0.45798
0.120969
0.896067
PPP*
1.32E-4
1.47E-3
2.80E-6
2.33E-5
3.93E-6
2.91E-5
r5p- >
4.38E-4
0.00486
6.84E-4
0.00570
0.0005799
0.004296
cit- > glt
3.50E-3
0.03884
2.13E-3
0.01775
0.0014090
0.010437
- > cit
0.00549634
0.06099
2.35E-3
0.01959
0.0023398
0.017332
Transketolase:






xu5p- > s7p***
0.00826654
0.09174
2.26E-7
1.88E-6
1.46E-6
1.08E-5
s7p- > xu5p
0.0084719
0.09402
4.24E-4
0.00353
0.0003579
0.002651
f6p- > xu5p
5.66E-4
0.00628
3.54E-5
0.00030
3.03E-5
0.000224
xu5p- > f6p***
5.38E-4
0.00597
3.45E-9
2.87E-8
1.89E-8
1.40E-7
f6p- > s7p
0.00534401
0.05930
2.46E-4
0.00205
0.0001979
0.001466
s7p- > f6p
0.0052113
0.05783
4.49E-5
0.00037
3.08E-5
0.000228
xu5p < - > g3p***
8.75E-4
9.71E-3
3.26E-8
2.71E-7
2.21E-7
1.64E-6
f6p < - > e4p
3.48E-4
0.00386
3.75E-6
3.12E-5
2.63E-6
1.95E-5
r5p < - > s7p
0.0800302
0.88812
2.94E-3
0.02453
0.0023626
0.017501
Transaldolase:






f6p- > s7p
1.54E-4
0.00171
2.57E-4
0.00214
0.0002003
0.001484
s7p- > f6p
2.48E-5
0.00028
2.25E-5
0.00019
4.54E-6
3.36E-5
f6p < - > g3p
2.64E-7
2.93E-6
1.09E-6
9.05E-6
2.51E-7
1.86E-6
s7p < - > e4p 0.0144573 0.16044 0.0053368 0.04447 0.0037384 0.027691

The fluxes were computed for cells incubated for 48 hours without drugs (control) or treated with either 0.5 μg × mL-1 of edelfosine that caused less than 1% of apoptosis ( 0-1%) or with 1.0 μg × mL-1 of edelfosine that caused 4-5% of apoptotis (4-5%). Left column for each condition shows absolute value of the flux (in mM × min-1 in intracellular volume), right column shows the values normalized per respective glucose uptake (hk). The flux depicted as TCA- > pyr is a sum of fluxes through pepck and malic enzyme, that resulted in the same isotopomer distribution and contribute to the 13C enrichment in lactate from the TCA cycle. Arrows indicate the directions of fluxes; stars indicate the fluxes for which the absolute values (not normalized) are statistically different from control (***, p < 0.01; **, p < 0.05; *, p < 0.1).