Table 3.
Simulated metabolic fluxes corresponding to the best fit presented in Table 2.
| flux | Control | Edelf: 0.5 μg/mL | Edelf:1 μg/mL | |||
|---|---|---|---|---|---|---|
|
hk*** |
0.0901118 |
1 |
0.12 |
1 |
0.135 |
1 |
| pfk |
0.0968532 |
1.07481 |
0.120985 |
1.00821 |
0.135572 |
1.004237 |
| fbpase |
0.00716729 |
0.07954 |
1.46E-3 |
0.01219 |
0.0009715 |
0.007197 |
| pep- > g3p |
0.00694225 |
0.07704 |
0.00749499 |
0.06246 |
0.0025560 |
0.018933 |
| g3p- > pep |
0.186074 |
2.06492 |
0.246177 |
2.05148 |
0.271442 |
2.010681 |
| pk |
0.181224 |
2.01110 |
0.253755 |
2.11463 |
0.28068 |
2.079111 |
| TCA- > pyr |
2.23E-3 |
0.02470 |
0.0152166 |
0.12681 |
0.0118396 |
0.087701 |
| lac- > |
0.12219 |
1.35598 |
0.153661 |
1.28051 |
0.128901 |
0.954822 |
|
pdh*** |
0.057984 |
0.64347 |
0.0815881 |
0.67990 |
0.140089 |
1.037696 |
| pc |
1.05E-3 |
0.01165 |
0.0185053 |
0.15421 |
0.0116895 |
0.086589 |
|
CitSyn** |
0.0351159 |
0.38969 |
0.0547404 |
0.45617 |
0.120038 |
0.889170 |
|
cit- > mal** |
0.0366362 |
0.40656 |
0.0549581 |
0.45798 |
0.120969 |
0.896067 |
|
PPP* |
1.32E-4 |
1.47E-3 |
2.80E-6 |
2.33E-5 |
3.93E-6 |
2.91E-5 |
| r5p- > |
4.38E-4 |
0.00486 |
6.84E-4 |
0.00570 |
0.0005799 |
0.004296 |
| cit- > glt |
3.50E-3 |
0.03884 |
2.13E-3 |
0.01775 |
0.0014090 |
0.010437 |
| - > cit |
0.00549634 |
0.06099 |
2.35E-3 |
0.01959 |
0.0023398 |
0.017332 |
| Transketolase: |
||||||
|
xu5p- > s7p*** |
0.00826654 |
0.09174 |
2.26E-7 |
1.88E-6 |
1.46E-6 |
1.08E-5 |
| s7p- > xu5p |
0.0084719 |
0.09402 |
4.24E-4 |
0.00353 |
0.0003579 |
0.002651 |
| f6p- > xu5p |
5.66E-4 |
0.00628 |
3.54E-5 |
0.00030 |
3.03E-5 |
0.000224 |
|
xu5p- > f6p*** |
5.38E-4 |
0.00597 |
3.45E-9 |
2.87E-8 |
1.89E-8 |
1.40E-7 |
| f6p- > s7p |
0.00534401 |
0.05930 |
2.46E-4 |
0.00205 |
0.0001979 |
0.001466 |
| s7p- > f6p |
0.0052113 |
0.05783 |
4.49E-5 |
0.00037 |
3.08E-5 |
0.000228 |
|
xu5p < - > g3p*** |
8.75E-4 |
9.71E-3 |
3.26E-8 |
2.71E-7 |
2.21E-7 |
1.64E-6 |
| f6p < - > e4p |
3.48E-4 |
0.00386 |
3.75E-6 |
3.12E-5 |
2.63E-6 |
1.95E-5 |
| r5p < - > s7p |
0.0800302 |
0.88812 |
2.94E-3 |
0.02453 |
0.0023626 |
0.017501 |
| Transaldolase: |
||||||
| f6p- > s7p |
1.54E-4 |
0.00171 |
2.57E-4 |
0.00214 |
0.0002003 |
0.001484 |
| s7p- > f6p |
2.48E-5 |
0.00028 |
2.25E-5 |
0.00019 |
4.54E-6 |
3.36E-5 |
| f6p < - > g3p |
2.64E-7 |
2.93E-6 |
1.09E-6 |
9.05E-6 |
2.51E-7 |
1.86E-6 |
| s7p < - > e4p | 0.0144573 | 0.16044 | 0.0053368 | 0.04447 | 0.0037384 | 0.027691 |
The fluxes were computed for cells incubated for 48 hours without drugs (control) or treated with either 0.5 μg × mL-1 of edelfosine that caused less than 1% of apoptosis ( 0-1%) or with 1.0 μg × mL-1 of edelfosine that caused 4-5% of apoptotis (4-5%). Left column for each condition shows absolute value of the flux (in mM × min-1 in intracellular volume), right column shows the values normalized per respective glucose uptake (hk). The flux depicted as TCA- > pyr is a sum of fluxes through pepck and malic enzyme, that resulted in the same isotopomer distribution and contribute to the 13C enrichment in lactate from the TCA cycle. Arrows indicate the directions of fluxes; stars indicate the fluxes for which the absolute values (not normalized) are statistically different from control (***, p < 0.01; **, p < 0.05; *, p < 0.1).