Table 2.
Summary and comparison of pre-miRNA base pairing and nucleotide composition
| Species | Conservation | bp % | (A-U) % | (A+U) % | A % | U % | G % | C % |
|---|---|---|---|---|---|---|---|---|
| Human | Sn (132) | 68.66 ± 7.83 | 42.73 ± 16.12 | 47.11 ± 13.18 | 22.10 ± 7.89 | 25.01 ± 6.96 | 27.49 ± 7.90 | 25.40 ± 7.24 |
| Sc1 (60) | 71.88 ± 6.20A | 49.34 ± 11.81Ab | 53.87 ± 10.66A | 23.78 ± 6.36 | 30.09 ± 6.42A | 24.45 ± 5.53AB | 21.68 ± 5.84A | |
| Sc2 (66) | 72.94 ± 5.64Ab | 48.67 ± 8.17AB | 51.62 ± 7.15AB | 23.41 ± 4.74B | 28.21 ± 4.42AB | 25.71 ± 3.49aB | 22.67 ± 5.11AB | |
| Sc3 (33) | 73.48 ± 4.67AB | 50.14 ± 11.02Ab | 52.94 ± 9.67a | 22.96 ± 6.05 | 29.98 ± 6.16A | 26.06 ± 5.15B | 20.99 ± 5.92A | |
| Mouse | Sn (108) | 67.87 ± 6.51 | 44.35 ± 11.20 | 49.30 ± 9.65 | 22.40 ± 6.16 | 26.90 ± 5.97 | 27.28 ± 5.57 | 23.42 ± 5.74 |
| Sc1 (63) | 72.53 ± 5.85A | 48.20 ± 11.47a | 53.07 ± 9.42a | 23.29 ± 5.42 | 29.78 ± 6.11A | 25.30 ± 5.28a | 21.62 ± 5.16a | |
| Sc2 (75) | 72.10 ± 5.87A | 47.70 ± 8.14aB | 50.57 ± 6.77B | 22.90 ± 4.57b | 27.67 ± 4.40b | 26.28 ± 3.72B | 23.15 ± 5.02 | |
| Sc3 (39) | 73.37 ± 5.13A | 50.85 ± 10.93A | 53.05 ± 9.15a | 22.89 ± 5.90 | 30.16 ± 5.51A | 26.06 ± 5.06 | 20.90 ± 5.53a | |
| Chicken* | Sn (21) | 68.24 ± 7.37 | 37.13 ± 17.43 | 44.17 ± 14.75 | 19.47 ± 8.79 | 24.69 ± 8.01 | 29.35 ± 6.00 | 26.48 ± 9.74 |
| Sc2 (40) | 70.89 ± 7.06 | 51.22 ± 7.19AB | 55.11 ± 4.99AB | 24.60 ± 4.14B | 30.52 ± 3.97AB | 24.80 ± 3.53AB | 20.08 ± 3.24aB | |
| Sc3 (19) | 73.08 ± 5.14a | 53.80 ± 7.56AB | 57.18 ± 5.72AB | 24.45 ± 5.26 | 32.74 ± 3.26AB | 23.94 ± 4.49A | 18.87 ± 3.33AB | |
Results represent the mean (± SD) of detailed information of base pairing and nucleotide composition. The number of pre-miRNAs within each set is indicated in brackets behind set name. * Sc1 for chicken was excluded as it contained fewer than five pre-miRNAs. Pair-wise comparisons of means (two-sided t-test) and variances (F-test) were performed between non-conserved (Sn) and conserved pre-miRNAs (Sc1, Sc2, and Sc3) from human, mouse, and chicken genomes. a mean value significantly different from that of Sn at the 0.05 FDR level. A mean value is significantly different from that of Sn at the 0.01 FDR level. b variance is significantly different from that of Sn at the 0.05 FDR level. B variance significantly different from that of Sn at the 0.01 FDR level.