Figure 5. The BRCT motif, but not the Ring finger domain, is required for MMS-induced BRCA1 downregulation.

A) Schematic view of the deletion constructs used in this study. B) HeLa cells were transfected with various expression constructs for BRCA1 and 2 days post-transfection, cells were treated with 200 µM MMS and harvested at the indicated times for immunoblotting to detect either endogenous BRCA1 or mutant forms using anti-BRCA1 or anti-GFP respectively. β-actin immunodetection was used as a loading control.