Table 3. Antibodies used for immunohistochemistry.

Antibodies	Species	Antigen	Source	Dilution (cryostat sections)	Dilution (free-floating sections)
sMCH	Rabbit	Salmon MCH	EA3922 [39]	1/200	1/2000
rMCH	Rabbit	Rat MCH	EA3922 [39]	1/200	-
NEI	Rabbit	Rat NEI	EA3922 [39]	1/200	1/2000
CART	Mouse	Rat CART	Dr Clausen, Danemark	1/1000	1/4000
CART	Rabbit	CART (61–102)	Phoenix Pharmaceuticals, USA	1/3000	-
Parvalbumin	Mouse	Human parvalbumin	Swant, Suisse	1/5000	1/10000

The specificity of the salmon MCH-AS was verified by liquid phase inhibition, dot blot, and immuno-affinity [42],[39] as well as by immunohistochemistry/in situ hybridization double labeling [43],[44]. The monoclonal CART-AS was generously provided by Dr Clausen (see acknowledgements).