Table 3. Antibodies used for immunohistochemistry.
Antibodies | Species | Antigen | Source | Dilution (cryostat sections) | Dilution (free-floating sections) |
sMCH | Rabbit | Salmon MCH | EA3922 [39] | 1/200 | 1/2000 |
rMCH | Rabbit | Rat MCH | EA3922 [39] | 1/200 | - |
NEI | Rabbit | Rat NEI | EA3922 [39] | 1/200 | 1/2000 |
CART | Mouse | Rat CART | Dr Clausen, Danemark | 1/1000 | 1/4000 |
CART | Rabbit | CART (61–102) | Phoenix Pharmaceuticals, USA | 1/3000 | - |
Parvalbumin | Mouse | Human parvalbumin | Swant, Suisse | 1/5000 | 1/10000 |