Skip to main content
. 2010 Mar 31;2(1):7–13. doi: 10.4047/jap.2010.2.1.7

Fig. 2.

Fig. 2

A: Proliferation of cord blood-derived outgrowth endothelial-like cells (left) and human bone marrow stromal cells (hBMSCs) (right) as indicated by absorbance of formazan at 490 nm after 48 hrs. B: Osteogenic differentiation of hBMSCs modulated by human cord blood-derived outgrowth endothelial-like cells (hCBOECs). Activity of alkaline phosphatase (left, unit: intensity of fluorescence). Production of osteocalcin (right, ng/mL). hBMSCs (1 × 104/cm2) and hCBOECs (1 × 104/cm2) were co-cultured with IMDM media, 1% FBS, 10-7 M dexamethasone and each PRP (100 µl/ml). Group A; activation with 10% CaCl2 and plateletpoor plasma (PPP), Group B; lysis with 0.1% Triton-X, Group C; activation with 142.8 U/ml thrombin and 4.3 mg/ml CaCl2, Group D; activation with shear force, 20 mg/ml collagen, 10 U/ml thrombin and 2 mM CaCl2. Values represent mean and standard deviation.