Table 2.

Ad35 viral vector packaging capacities.

E1 deletion, transgene	Other genome modifications	pIX expression	Genome size (percent of wt)	Stability at Passage 3
d2, no transgene	None	No	94.92	Stable
d2, green fluorescent protein (GFP)	None	No	96.96	Stable
Δ447 - 3326, GFP	None	No	97.22	Stable
d2, secretory alkaline phosphatase	None	No	99.33	Rearrangements
d2, luciferase	None	No	99.79	Rearrangements
d2, bacterial beta-glucuronidase	None	No	99.94	Rearrangements
d2, HIV Env gp140ΔCFIΔV1V2	None	No	99.95	Rearrangements
d2, HIV Env gp140ΔCFI	None	No	100.41	Rearrangements
d2, lacZ	None	No	103.68	Rearrangements
d8, eGFP	E3(HE), E4(dORF3-6)	Yes	85.60	Stable
d8, eGFP	E3(HE), E4(dAN)	Yes	87.30	Stable
d5^, HIV Env gp140ΔCFIΔV1V2	None	Yes	100.43	Stable
d8, HIV Env gp140ΔCFI	None	Yes	101.70	Stable
d6, Luciferase	None	Yes	103.60	Stable**
d8, HIV Env gp140ΔCFI	Fiber modified*	Yes	103.90	Rearrangements
d8, Luciferase	Fiber modified*	Yes	104.11	Rearrangements

^d2 with AAV P5 promoter for pIX expression

*Ad35 fiber shaft and knob replaced with Ad5 fiber shaft and simian Ad25 knob

** Through greater than 5 passages