Table 1.
Summary of 28 sequenced clones. Sequencing of plasmid clones recovered after repair of the NHEJ- red cassette.
| Sequence | N | % | Deletion |
|---|---|---|---|
| HiFi TTATAA | 14 | 50 | none |
| Non-HiFi | |||
| ---ATAA | 5 | 17.9 | TT |
| -TATAA | 2 | 7.1 | T |
| TTAT--- | 2 | 7.1 | AA |
| TTATA- | 2 | 7.1 | A |
| TTA---A | 2 | 7.1 | AT |
| TTA---- | 1 | 3.6 | TAA |
DNA sequencing reveals the modifications to the repair site after Ad-SceI infection. The sequence obtained at the repair site, the missing nucleotides, as well as the frequency of the type of DNA damage from twenty-eight clones is shown.