Table 3. Complementation of V(D)J recombination driven signal and coding joint formation in SCID fibroblasts transfected with WT RAG-1 cDNA.
| Signal (pGG49) | Coding (pGG51) | |||||
|---|---|---|---|---|---|---|
| Cell lines | AmpR colonies | AmpR/CamR colonies | Ratea (%) | AmpR colonies | AmpR/CamR colonies | Rateb (%) |
| 17001 Exp 1 | 11 000 | 20 | 0.18 | 4000 | 12 | 0.30 |
| Exp 2 | 8000 | 12 | 0.15 | 5340 | 6 | 0.11 |
| Exp 3 | 7600 | 16 | 0.20 | 7600 | 8 | 0.105 |
| 17005 Exp 1 | 10 400 | 32 | 0.30 | 5000 | 12 | 0.24 |
| Exp 2 | 8700 | 15 | 0.17 | 4030 | 9 | 0.22 |
| Exp 3 | 9400 | 17 | 0.18 | 3000 | 10 | 0.33 |
AmpR, Ampicillin resistance; CamR, Chromphenicol resistance.
a
Rate (signal joints)=(AmpR/CamR colonies)/(AmpR colonies) × 100%.
b
Rate (coding joints)=AmpR/CamR colonies)/(AmpR colonies) × 100%.