Table 1. Data on CUGBP binding to mut RNA (see text for citations).

Data on CUGBP binding	Observation supports?		Comment
Biochemical data on CUGBP In vivo
Coimmunoprecipitation with mut RNA	Yes		Ab to CUGBP recovers mut RNA
HMW complexes by SEC	Yes		All CUGBP in mut RNA complex
Increased effect with longer repeat	Yes		Prolonged CUGBP half-life, accumulation, increased splicing abnormalities
Increased CUGBP (pharmacodynamics)	Yes		Level as predicted by mut RNA binding
Increased effect with high levels of short repeat	Yes		CUGBP accumulation, splice abnormalities, phenotypic DM
In vitro
Increased binding with longer repeat	Yes	No	For yes, max n=123; RNA-free
			For no, max n=90; RNA-blocked
Morphological data on CUGBP
In vitro
Binding to mut RNA	Yes		By EM, binds to ss CUGn regions
In vivo
Colocalization with mut RNA foci		No	Diffuse nuclear distribution for CUGBP