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. 2009 May 27;17(11):1454–1462. doi: 10.1038/ejhg.2009.80

Figure 2.

Figure 2

Titration of −308G/GL3Luc and −308A/GL3Luc DNA with or without the human TNF untranslated region (3′UTR). Jurkat cells were transiently transfected with varying amounts of reporter DNA (5, 3, 1, 0.5, and 0.1 μg) and harvested after 24 h to assay for luciferase activity using the Dual-Lucifrase reporter assay system (Promega). Reporter plasmids that were used include (a) −308G/GL3Luc and −308A/GL3Luc without 3′UTR or (b) −308G/GL3Luc and −308A/GL3Luc in context of the 3′UTR. Experiments were performed in triplicate. Values represent fold difference of raw luciferase relative light units (RLU) from −308A/GL3Luc over corresponding −308G/GL3Luc.