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. 2009 May 27;17(11):1454–1462. doi: 10.1038/ejhg.2009.80

Figure 3.

Figure 3

Effects of co-transfection with pRL-TK control plasmid. Represents the fold difference in transcriptional activity of −308A/GL3Luc and −308G/GL3Luc after normalizing RLU/s to −308G/GL3Luc only, which was set at 1. Jurkat cells were transiently co-transfected with biallelic −308 TNF reporter constructs along with different amounts of pRL-TK (30, 100, 300, 500 ng, and 2 μg) and harvested after 24 h to assay for luciferase activity using the Dual-Luciferase reporter assay system (Promega). The experiment was performed twice in triplicate. Values represent fold difference of raw luciferase RLU/s from −308A/GL3Luc over corresponding −308G/GL3Luc. Statistical significance was determined by Student's unpaired t-test. P<0.05 was considered significant.