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Comparative analysis of polymorphic −308 TNF promoter sequences. (a) Representation of both the −308G TNF and −308A TNF allelic promoter/GFP reporter constructs. (b) Both reporters were integrated into the FRT site of the FRT-Jurkat line and GFP expression assessed by flow cytometry. For cells carrying the −308G reporter, 34.5% of the cell population expresses GFP, whereas for the −308A reporter, 79.3% of the cell population expresses GFP. MF, mean fluorescence.
