Skip to main content
. 2003 Jan 22;100(3):880–885. doi: 10.1073/pnas.232735599

Figure 4.

Figure 4

IMAC phosphopeptide selection from a tryptic digest of GST-NPR1. (A) MALDI-TOF spectrum of phosphopeptides in linear mode. *, Number of phosphates associated with the peptide. White circles mark unspecifically bound peptides. (B) MALDI-TOF spectrum of the phosphopeptides eluted from the IMAC beads in the presence of alkaline phosphatase. The spectrum was acquired in reflector mode. (Inset) Coomassie blue-stained SDS gel of purified GST-NPR1 without (−CIP) or with (+CIP) alkaline phosphatase treatment.