Figure 2.

Upstream region of the Hexokinase1 (HK1) gene. (a) Structure of the 5′UTR of human and mouse HK1, summarising published information. The positions of the two nucleotide substitutions in Hereditary Motor and Sensory Neuropathies – Russe (HMSNR) chromosomes are marked with arrows. (b) HK1 transcripts identified in this study in mouse tissues using RT-PCR with forward primer in AltT2 and reverse primers in T3 and invariable exon 2 (See Supplementary Table 1 for primer sequences). The translation start site in exon T3¹⁸ is marked with an asterisk. The diagram illustrates the exon composition of alternative transcripts, with the corresponding fragments and sizes in bp shown in the agarose gel picture. Please refer to Supplementary Material online for the sequences of the different fragments.