Table 1. Quantitative RT-PCR primers that were used to study RNA expression in samples obtained from FSHD patients and controls.
| Primer | Sequence | Efficiency (%) | Correlation coefficient | Product size (bp) | cDNA input (ng) |
|---|---|---|---|---|---|
| Fw_ANT1 | 5′-TGCCTACTTCGGAGTCTATGATACTG-3′ | 99 | 0.992 | 84 | 5 |
| Rev_ANT1 | 5′-GCAATCATCCAGCTCACAAAAA-3′ | ||||
| Fw_FRG2 | 5′-GGGAAAACTGCAGGAAAA-3′ | 107 | 0.985 | 69 | 20 |
| Rev_FRG2 | 5′-CTGGACAGTTCCCTGCTGTGT-3′ | ||||
| Fw2_LRP2BP | 5′-GGGCTGGGGACCACTCTA-3′ | 104 | 0.939 | 78 | 20 |
| rev2_LRP2BP | 5′-TGCTTTGGGACATGGAGAAT-3′ | ||||
| Fw1_ALP | 5′-CTCAGGGGGCATAGACTTCA-3′ | 101 | 0.998 | 91 | 5 |
| rev1_ALP | 5′-ATCTCCAGGACACAGGTTGG-3′ | ||||
| PITX1 forw | 5′-ACATGAGCATGAGGGAGGAG-3′ | 89 | 0.999 | 107 | 5 |
| PITX1 rev | 5′-GTTACGCTCGCGCTTACG-3′ | ||||
| FRG1(4)taqF | 5′-GGCGGGTTCTACAGAGACG-3′ | 111 | 0.981 | 103 | 5 |
| FRG1(4)taqR | 5′-TTCTGGACGAGTATGTGAGTCG-3′ | ||||
| humCRYM forw | 5′-GCTGGGAGAAGTGATTAAGG-3′ | 97 | 0.98 | 104 | 20 |
| humCRYM rev | 5′-TGAGTTTGGCTGCAACTGTG-3′ | ||||
| hGAPDHFw | 5′-AGCACATCGCTCAGACAC-3′ | 97 | 0.999 | 65 | 5/20 |
| hGAPDHRev | 5′-GCCCAATACGACCAAATCC-3′ |
All primer sets spanned at least one intron. Efficiency of each primer pair, the correlation coefficient as well as the expected product size are indicated.