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Effect of TBPm3 on transcription. (A) Transcriptional activity of TGTA-containing duplex and −6 o/h promoters was tested in the presence of TFIIIB assembled with wild-type TBP (lanes 1–6) or TBPm3 (lanes 7–12). Template nomenclature follows Fig. 2A. (B) Transcriptional activity in the presence of TBP (filled bars) or TBPm3 (open bars) relative to duplex DNA. Error bars are SEM of at least three independent experiments.
