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Transcription with mutant TFIIIB subunits. Transcription of the indicated 5′ overhang templates and duplex DNA was carried out in the presence of wild-type TFIIIB (wt), TFIIIB assembled with Bdp1(Δ355–372) substituting for Bdp1 (Bdp1Δ), or Brf1NΔ68 substituting for Brf1 (Brf1Δ). Lanes 1–3 and 4–24 are from separate experiments.
