Figure 4.

GAG synthesis in fibroblasts transfected with indicated siRNAs. Fibroblasts were transfected with indicated siRNAs, and after 48 h cultivation the transfection was repeated and cultivation was prolonged for another 24 h. GAG synthesis was estimated by measuring the incorporation of ³⁵S as described in the experimental procedures. The presented values are the means of three independent experiments with corresponding SD. siRNAs for XYLT1 (siRNA ID# 112165, siRNA ID# 29873), XYLT2 (siRNA ID# 112168, siRNA ID# 212974), GALTI (B4GALT7) (siRNA ID# 19329, siRNA ID# 111770) and GALTII (B3GALT6) (siRNA ID# 112321, siRNA ID# 112322) genes were used. ‘Mock control' indicates conditions under which only a buffer was used in transfection-like experiments (without any siRNA), and ‘negative control' represents results with AllStars Negative Control siRNA (see experimental procedures for details). ^*P<0.05 compared with the no siRNA control.