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. 2009 Aug 19;18(2):194–199. doi: 10.1038/ejhg.2009.143

Figure 1.

Figure 1

Quantitative knockdown of EXTL2 and EXTL3. 293T cells were transfected with psiCHECK-2EXTL2 or psiCHECK-2EXTL3 only, or co-transfected with a psiCHECK-2 target gene construct and corresponding shEXTL or ctrlEXTL expressed from the pENTR/U6 vector. Control cells were mock transfected with lipofectamine 2000 reagent only. A DLR assay was conducted 24 h post-transfection, and results expressed as a percentage of psiCHECK-2 target gene expression. Results are the mean±standard deviation of n=10 replicates. *Significant difference from mock transfection P<0.001 (ANOVA, Tukey's HSD).