Figure 5.

Expression of shRNAs from a lentiviral vector. 293T cells were transfected with pHIV-1shEXTL2.1, pHIV-1shEXTL2.2, pHIV-1shEXTL2.3, pHIV-1shEXTL3.2 or pHIV-1ctrlEXTL, and a DLR assay was performed to determine whether shRNAs were expressed from the vector (a). 293T cells were also transduced with virus encoding the same shRNAs and the rate of gag synthesis measured 7 days after transduction (b). Results are the mean±standard deviation of three replicates and are expressed as the percentage of mock control. ^*Significant difference from mock transfection P<0.001 (ANOVA, Tukey's HSD).