Table 1. shRNA and target gene homologous sequences.
shRNA | Target gene homologous sequence (5′–3′) | Site of target sequence | Accession number |
---|---|---|---|
shEXTL2.1 | GCAGCAATTTCCTGATCAAAT | 788–808 | AF000416 |
shEXTL2.2 | GCAAGACTTCAGGGATATTTG | 1057–1077 | AF000416 |
shEXTL2.3 | GGTGTTGCCACATCTGCAAAC | 292–312 | AF000416 |
shEXTL3.1 | GCTTTCTCTGGGAGACTTACT | 2139–2159 | AF001690 |
shEXTL3.2 | GGTGGTGATGTTGACTTATGA | 2593–2613 | AF001690 |
shEXTL3.3 | GCTCAGCTTCACGCTCTTTGT | 694–714 | AF001690 |
Six shRNA oligos were designed, each with sequence homology to a different target sequence within the EXTL2 or EXTL3 gene. The position of each shRNA within the target gene is specified.