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. 2003 Jan 27;100(3):1034–1039. doi: 10.1073/pnas.0237312100

Figure 3.

Figure 3

Smad4dn inhibits tumor growth in vivo. (a) Nude mice were injected s.c. with GH3 cells stably transfected with an empty vector (GH3–vector) or with a vector expressing Smad4dn (GH3–Smad4dn). At 30 days after injection, large tumors formed by GH3–vector cells were observed and compared with the smaller tumors in animals injected with GH3–Smad4dn cells. (b) Tumors of animals injected as indicated in a were detected 15 days after injection, and growth was monitored for a total of 30 days. ♦, GH3–vector; ○, GH3–Smad4dn. These results represent two independent experiments in which three and four animals, respectively, were injected with each cell line. (c) c-Myc and Smad4dn (FLAG) expression was analyzed by Western blot in tumor samples from nude mice injected with GH3 cells stably transfected with an empty vector (control tumor growth) or Smad4dn-expressing GH3 cells (inhibited tumor growth) after 30 days. Two additional tumor samples that escaped from the initial Smad4dn inhibition and presented a late increase in tumor size (late tumor growth) were analyzed for c-Myc and Smad4dn (FLAG) expression by Western blot. Equal loading was assessed by β-actin detection.