Figure 4.

Steady-state levels and conformation of tRNA^Trp and tRNA^Arg. Fibroblasts and cybrid clones from patient T (P_T) and A (P_A) and controls (C1–3 and 143B) were analyzed using high-resolution northern blot under denaturing (steady-state) and native (conformation) conditions. Signal intensities for tRNA^Trp and tRNA^Arg were normalized to each other for each sample, as shown in the column charts (only relevant steady-state levels are depicted). The asterisks indicate the position of altered conformations of mutant tRNA^Trp and mutant tRNA^Arg.