Figure 2.

Effect of CHX treatment on the level of ELN mRNA. (a, d) Schematic representation of wild-type, c.1161delC, c.1195delG, and c.838_839insG minigenes, respectively. CMV promoter is marked by a thick horizontal arrow. Boxes indicate coding regions and horizontal thin lines represent introns. The position of mutations is indicated with a star. (b, c, and e) The level of mRNA transcribed from different ELN constructs was normalized to the mRNA level of GFP. The ratio between these normalized levels after CHX treatment was calculated and compared with the ratio in untreated cells. The fold increase in the level of indicated ELN transcripts is shown as mean±SEM.