FIG. 2.

Proinflammatory cytokine production was measured after LPS stimulation of splenic macrophages. Spleens were harvested at 0.5, 3, and 6 h after a single dose of ethanol at 1.2 g/kg (striped bars), or 2.9 g/kg (filled bars), or saline (open bars) as a control. Splenic macrophages were purified as described in Materials and Methods, and stimulated with 100 ng/mL LPS for 16 h. (A) TNF-α, (B) IL-6, and (C) IL-12 were measured by ELISA. Open bars represent saline group, shaded bars represent group treated with 1.2 g/kg ethanol, and filled bars represent group treated with 2.9 g/kg ethanol. Data are shown as mean ± SEM. N = 3–6 animals. *p < 0.05 from saline control group.