FIG. 4.

Phagocytosis of enhanced green fluorescent protein (EGFP)–P. aeruginosa by alveolar macrophages was measured using fluorescence microscopy. Alveolar macrophages were incubated with EGFP–P. aeruginosa for 30 min after they were isolated from saline-treated or ethanol-treated (2.9 g/kg) mice. Cells were fixed, and the fluorescent intensity of EGFP–P. aeruginosa (green) was used to calculate phagocytosis after staining with rhodamine phalloidin (red). Figure shows representative images of alveolar macrophages isolated from (A) saline-treated and (B) ethanol-treated mice. Phagocytic index of alveolar macrophages isolated from (C) mice treated with saline or ethanol. Data are shown as mean ± SEM. N = 3–4 animals. *p < 0.05 from saline control group.