FIG. 3.

IFN-induced gene expression. Cells were stimulated with IFN-β or IFN-γ 1 h after infection or left unstimulated for a total of 5 h, and RNA or protein were isolated. Equal amounts of RNA were used to create cDNA for quantitative PCR. Gene expression was determined in duplicate and reported as an average ± SD. (A) Immunoblots of 5-h infection of BMDM. Uninfected or VACV-infected BMDM were incubated with or without IFN-γ or IFN-β for the last 4 h of a 5-h infection. Whole cell extracts were separated by SDS-PAGE and immunoblotted for phospho-Stat1 or phospho-Stat2. Immunoblots were stripped and reprobed for total Stat1 or total Stat2. Data are representative of two experiments. (B) IFN-induced gene expression. Gene expression in the presence or absence of the indicated IFN is shown in uninfected BMDM (upper panel) or VACV-infected BMDM (lower panel). Gene expression was determined in duplicate and reported as an average ± SD. Data are representative of three experiments.