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(A) Monolayers were prepared to present both the enzyme substrate and the adaptor ligand against a background of tri(ethylene glycol) groups. The enzyme converts the substrate to a hydroquinone product, which can be detected with cyclic voltammetry. SAMDI mass spectra of the monolayer confirm the presence of the valerate substrate before treatment with the enzyme (B) and the hydroquinone product following incomplete treatment (C).
