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. 2010 Jan 20;18(6):713–719. doi: 10.1038/ejhg.2009.239

Figure 2.

Figure 2

The pFlag–Neo–Siglec-8 plasmid construct and Siglec-8 expression on Siglec-8-transfected HEK293 cells. (a) The pFlag–Neo–Siglec-8 plasmid constructs contains either the SNP rs10409962 risk (A allele) or protective allele (G allele). (b) Detection of Siglec-8 expression on Siglec-8-transfected HEK293 cells by means of flow cytometry. Cells were analyzed for specific staining with isotype (mouse IgG), Siglec-8 antibody (2C4), and Siglec-8 ligand (6′-sulfo-sLex polymer) in HEK293 cells non-transfected (top), transfected with Siglec-8 WT (middle), and transfected with the Siglec-8 Ser-to-Pro mutation (bottom). The data represent one of three experiments. Values in the upper right corner of each histogram represent percent positive.