Figure 3.
Verification of gene predictions by RT-PCR analysis. (A and B) Test of prediction 6F5, a homolog of Drosophila brain-specific homeobox protein (bsh). (C and D) Test of prediction 11F6, a homolog of rat vanilloid receptor type 1-like protein. Gel analysis of amplimers (*) with the source of the cDNA pool indicated above is shown in A and C. Primers (blue) and the region to which the amplimer sequence aligned (underlining) are shown in B and D. The indicated forward primers were used to generate the amplimer sequences (brain amplimer, B; skin amplimer, D). Br, brain; Ey, eye; He, heart; Ki, kidney; Li, liver; Lu, lung; Mu, muscle; Ov, ovary; Sk, skin; St, stomach; Te, testis; Th, thymus.