Table 1.

Predicted novel gene sets and RT-PCR verification rates

Pool	Programs*	No. of predictions	No. tested	No. positive	Success rate, %	Expected successes	Standard error
Enriched†	Both	827	154	117	75.97	628
	One	601	60	16	26.67	160
	Total	1,428	214	133	62.15	788	48
Similar‡	Both	505	16	4	25.00	126
	One	1,620	22	0	0.00	0
	Total	2,125	38	4	10.53	126	105
Other§	Both	234	5	1	20.00	46
	One	3,425	58	1	1.72	59
	Total	3,659	63	2	3.17	105	83
All	Total	7,212	315	139	N/A	1,019

N/A, not applicable. 

^*Both, Genes predicted at least partially by both twinscan and sgp2 programs. One, Genes predicted by one program that are not overlapped by predictions of the other program. N/A, not applicable. 

^†Mouse gene predictions containing an intron whose flanking exonic regions align with flanking exonic regions predicted by the same program in human. 

^‡Mouse gene predictions that fail the enrichment step but show regions of strong similarity to a gene predicted by the same program in human. 

^§Mouse gene predictions without regions of strong similarity to any gene predicted by the same program in human.