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. 2010 May 5;18(9):985–992. doi: 10.1038/ejhg.2010.61

Figure 4.

Figure 4

Subcellular localization of mutant C × 47 in HeLa cells. Communication incompetent HeLa cells transiently transfected with mutant C × 47 (T398I, G149S, A98G_V99insT, T265A, and G236R) were immunolabeled with a rabbit antiserum against human C × 47 (green) and a mouse monoclonal antibody against the ER protein disulfide isomerase (α PDI, red). Gap junctional plaques were seen at the plasma membrane for cells expressing mutant T398I and G149S (a and b). There was also partial co-localization with the ER marker for both mutants, but the ER co-localization was even higher for the mutant G149S. Nearly perfect ER co-localization was seen for the mutants A98G_V99insT and T265A (c and d). As expected, there was no signal for mutant G236R (e).