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. 2010 Nov 9;123(23):4128–4144. doi: 10.1242/jcs.072041

Fig. 5.

Fig. 5.

Kazrin and ARVCF localization in desmosome. (A–I) Human keratinocytes were immunolabeled for the endogenous desmosomal marker desmoplakin (A,E), Myc-tagged xARVCF (B), or endogenous Kazrin (D,G). In addition, Kazrin localization was compared to that of endogenous desmoglein3 (H), a desmosomal cadherin. Merged images (F,I) show partial colocalization of Kazrin with desmoplakin and extensive colocalization with desmoglein3. Colocalization between desmoplakin and ARVCF is shown in C. Photos represent projection images of deconvolved Z-stacks. Scale bars: 10 μm. (J) Interaction between desmosomal cadherin desmoglein1 (Dsg1) and xARVCF in Xenopus embryo lysates. Indicated amounts of in vitro transcribed RNAs were microinjected into one-cell stage embryos. Then, early gastrula embryos were lysed and human desmoglein1 tail or xARVCF were immunoprecipitated, followed by SDS-PAGE and western blotting. (K) Endogenous desmoglein1 or E-cadherin were immunoprecipitated from confluent A431 human epithelial carcinoma cells. Bound endogenous ARVCF was detected with human ARVCF-specific antibody.