Table 2.
Amino acid sequences of TCRβ CDR3 sequences from individual CD4+ T cell clones identified using the clonotypic assay
| Donor/Pt. pair no. | CDR3 sequence of dominant alloreactive clones
|
CDR3 sequence of dominant leukemia-reactive clones
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total no. of sequences | Total no. of clones | Sequence frequency of alloreactive clones, % | TCRβV | TCRβD | TCRβJ | Total no. of sequences | Total no. of clones | Sequence frequency of leukemic clones, % | TCRβV | TCRβD | TCRβJ | |
| 1 | 55 | 36 | 12 (22) | QGDSAMYLCASS* | PE | AFFGQGTRLTVV | 53 | 26 | 24 (45) | PEDSSFYICSA | SGAD | SGANYLTFGAGSRLTVL |
| 6 (11) | LGDSALYLCASS | DGTGD | EQFFGPGTRLTVL | 4 (8) | LGDSALYLCASS | GTSAS | SYNEQFFGPGTRLTVL | |||||
| 3 (6) | LGDSALYLCASS | GTSAS | SYNEQFFGPGTRLTVL | 2 (4) | PSQTSVYFCASS | PGTG | EQFFGPGTRLTVL | |||||
| 2 (4) | LEDSAMYFCAS | RAPAT | YNSPLHFGNGTRLTVT | |||||||||
| 2 | 51 | 51 | 1 (2) | Polyclonal† | 46 | 36 | 6 (13) | RGDSAAYFCASS | RRTGSG | NEQFFGPGTRLTVL | ||
| 4 (9) | LEDSAMYFCASS | REAF | YGYTFGSGTRLTVV | |||||||||
| 2 (4) | QEDSAVYLCASSL | AGTGS | SGANVLTFGAGSRLTVL | |||||||||
| 2 (4) | PNQTSLYFCASSL | QGVP | EAFFGQGTRLTVV | |||||||||
| 3 | 47 | 47 | 1 (2) | Polyclonal | 49 | 28 | 10 (20) | TNQTSMYLCASI | TRRAG | QYFGPGTRLTVT | ||
| 8 (16) | LGDSALYLCASS | FPGTSG | YNEQFFGPGTRLTVL | |||||||||
| 3 (6) | QDSAMYRCASS | PTMREGE | NEQFFGPGTRLTVL | |||||||||
| 3 (6) | LGDSALYLCASSQ | GS | GYTFGSGTRLTVV | |||||||||
| 2 (4) | PSQTSVYFCAS | GPRPL | ETQYFGPGTRLLVL | |||||||||
| 4 | 49 | 49 | 1 (2) | Polyclonal | 50 | 44 | 5 (10) | PEDSALYLCASSQ | GTGV | NEQFFGPGTRLTVL | ||
| 2 (4) | RGDSAVYLCAS | PGQ | SYNEQFFGPGTRLTVL | |||||||||
| 2 (4) | QEDSAVYLCASSL | RLAG | TDTQYFGPGTRLTVL | |||||||||
| 5 | 50 | 49 | 2 (4) | PEDSSFYICSAR | GTG | NEKLFFGSGTQLSVL | 52 | 34 | 12 (23) | PRDSAVYFCASSL | ALTRGREP | GELFFGEGSRLTVL |
| 1 (2) | Polyclonal | 4 (8) | LEDSAMYFCASS | ATGGLR | YNEQFFGPGTRLTVL | |||||||
| 4 (8) | PSQTSVYFCASSD | RYA | TQYFGPGTRLLVL | |||||||||
| 2 (4) | LEDSAMYFCAS | RTGT | NTGELFFGEGSRLTVL | |||||||||
| 6 | 54 | 54 | 1 (2) | Polyclonal | 52 | 37 | 9 (17) | PNQTALYFCATS | QGAV | YEQYFGPGTRLTVT | ||
| 7 (13) | LGDSALYLCASS | PILD | GELFFGEGSRLTVL | |||||||||
| 2 (4) | LGDTAMYLCAT | RQRAG | EKLFFGSGTQLSVL | |||||||||
| 7 | 51 | 51 | 1 (2) | Polyclonal | 54 | 38 | 14 (26) | PSQTSVYFCASS | RTLG | YEQYFGPGTRLTVT | ||
| 4 (7) | SSQTSVYFCAI | VAEWRTS | NEQFFGPGTRLTVL | |||||||||
| 8 | 50 | 50 | 1 (2) | Polyclonal | 46 | 32 | 10 (22) | PNQTSLYFCASSL | FRLASGT | SYEQYFGPGTRLTVT | ||
| 4 (9) | LGDSALYFCASS | VVKGLGD | EQFFGPGTRLTVL | |||||||||
| 2 (4) | RGDSAAYFCASSP | RSGS | TGELFFGEGSRLTVL | |||||||||
| 2 (4) | PSHTSQYLCASSE | GPVE | QYFGPGTRLTVL | |||||||||
| 9 | 48 | 48 | 1 (2) | Polyclonal | 49 | 37 | 7 (14) | TNQTSMYLCASSL | YGDRGH | GYTFGSGTRLTVV | ||
| 5 (10) | QGDSAMYLCASS | SRGGS | TGELFFGEGSRLTVL | |||||||||
| 3 (6) | LGDSAVYFCASS | GEI | SGNTIYFGEGSWLTVV | |||||||||
*
Oligoclonal: two or more TCRβ V(D)J sequences are the same in two or more individual clones.
†
Polyclonal: each of the TCRβ V(D)J sequences in 47–54 clones is different.