Fig. 5.
Analysis of the deletion/substitution mutations introduced into the apical region of SL2. (a) Part of the RNA secondary structure depicting SL2 with the region in which mutations were introduced is highlighted by an unbroken-line box. (b) Nature of the deletion/substitution and destabilization/compensatory mutations that were introduced into the upper region of SL2. The precise nature of each mutation is shown separately along with the adjoining area of the RNA secondary structure. (c) RPEs of transfer vector RNAs containing mutations in the upper region of SL2. (d) Relative transfer vector RNA propagation for each mutant expressed as Hygr CFU/ml of the viral supernatant. The RPE and propagation data are derived from at least three independent transfection and infection experiments after normalization to the transfection efficiency.