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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Pharm Res. 2010 Sep 25;27(12):2766–2775. doi: 10.1007/s11095-010-0278-4

Figure 2.

Figure 2

Comparative expression of oxidative stress response and antioxidant defense genes in PC-3 and PrEC cells. A) Relative expression of genes involved in oxidative stress response and antioxidant defense between PrEC and PC-3 cells. Genes up- and down-regulated are represented by red and green dots, respectively. Scatter plot shows a log transformation of the relative expression level of each gene between PC-3 and PrEC cells. B) Cluster analysis demonstrating differences in gene expression between PC-3 and PrEC cells. Genes represented in the gene cluster analysis are limited to those whose expression differs between the cells by at least 2-fold. Two independently prepared samples of each cell line in duplicate were used for gene expression profiling (n=4). C) Immunoblotting for NOX5 and FOXM1 using two lysates from PrEC and PC-3 cells. Membranes were stripped and re-probed with anti-actin antibody to ensure equal protein loading.