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. Author manuscript; available in PMC: 2011 Nov 17.
Published in final edited form as: Bioconjug Chem. 2010 Oct 6;21(11):2065–2075. doi: 10.1021/bc100288c

Scheme 3.

Scheme 3

(A, B) Glycan-patterned slides by microcontact printing of BSA glycoconjugates onto NHS-activated glass substrates, followed by a blocking step. (C) Patterned capture slides were exposed to Pseudomonas at variable concentrations, then imaged under darkfield condtions. (D) Image processing by FFT analysis produced reciprocal lattice peaks at k=±1/a in spectral format; central peak (k=0) scales with spatially averaged intensity of original image; noise from nonspecific binding is dispersed throughout k-space.