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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nature. 2010 Jul 22;466(7305):451–456. doi: 10.1038/nature09291

Figure 1. Specific fat cell gene dysregulation by the cdk5-mediated S273 phosphorylation of PPARγ.

Figure 1

a, In vitro CDK assays performed using cdk5/p35 with either wild type (WT) or S273A mutated PPARγ. b, Phosphorylation of PPARγ in differentiated 3T3-L1 adipocytes stimulated with TNF-α for the indicated times. c, Phosphorylation of PPARγ in cells expressing scrambled or CDK5 shRNA stimulated with indicated cytokines. NT, no treatment. d, Staining of PPARγ-null fibroblasts expressing WT or S273A mutant PPARγ with Oil-Red-O. e, Gene expression in these cells was analyzed by real-time quantitative PCR (qPCR) for expression of various genes (n=3). f, mRNA expression in transplanted fat pads was analyzed by qPCR (n=5) (Error bars are s.e.m.; *p<0.05, **p<0.01).